Astuti, Tri and Ali Akbar, Syahro and Rofiq, M. Nasir and Jamarun, Novirman THE EVALUATION OF ENZYME ACTIVITY OF CELLULASE, LIGNINASE, AND IDENTIFICATION OF MICROBIAL MORPHOLOGY AT LOCAL BIOACTIVATOR FROM CATTLE AND BUFFALO RUMEN CONTENT WITH DIFFERENT ENERGY SOURCES (Article Submit). [Artikel Dosen]
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Abstract
This study aims to determine the activity of cellulase and ligninase enzymes on local bioactivator. This was based on the rumen contents of the rumen incubated for seven days with supplies of different sources of enzyme energy. The method used was a factorial design of 2 x 4 with three replications for each treatment. Factor A was the type of Animal (Cattle Vs Buffalo), factor B is the addition of an enzyme source supply material: B1 = Molasses, B2 = Molasses + palm frond extract, B3 = Molasses + palm leaf extract, B4 = Molasses + palm frond, and palm leaf extract. Also, the parameters observed were the activity of enzymes cellulase, laccase, lignin and manganese peroxidase, as well as microbial identification. The results of statistical analysis showed that the activity of the enzymes cellulase 2,22-3,51 U/ml, laccase 10,62- 20,11 U/ml, lignin peroxidase 1,74-4,93 U/ml, and manganese peroxidase 2,40-7,06 U/ml showed insignificant differences (p> 0.05). The results of microbial identification found the bacteria Lactobacillus sp. Based on these, it was concluded that the microbes discovered in the local microorganism solution live because of the study environment, not the microbes inherited from the rumen's contents.
| Item Type: | Artikel Dosen |
|---|---|
| Subjects: | Agriculture > S Agriculture (General) |
| Depositing User: | Tri Astuti |
| Date Deposited: | 06 Jun 2023 01:38 |
| Last Modified: | 06 Jun 2023 01:38 |
| URI: | http://repository.ummy.ac.id/id/eprint/435 |
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