Astuti, Tri and Ali Akbar, Syahro and Rofiq, M. Nasir and Jamarun, Novirman and Huda, Nurul and Fudholi, Ahmad (2022) The Activity of Cellulase and Ligninase Enzymes In Local Bioactivator From Cattle And Buffalo Rumen Content. Biocatalysis and Agricultural Biotechnology.
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Abstract
Lignin is the main component of agricultural and plantation waste such as bagasse, straw, and oil palm fronds. Lignin, cellulose, and hemicellulose in lignocellulosic bonds can be broken down by enzymes. There are many types of research that utilize plantation waste as feed ingredients, but lignin is a limiting factor that affects the digestibility of these materials, so their content must be reduced before being used as feed ingredients. Using of local bio enzymes will be effective in breaking lignocellulose bonds. However, it is necessary to find sources of enzymes that are easy to obtain, inexpensive to produce, and effective as lignocellulosic degrading enzymes. This study aims to determine the activity of cellulase and ligninase enzymes on local bio activators. This was based on the rumen contents of the rumen incubated for seven days with supplies of different sources of enzyme energy. This treatment used rumen content of Cattle and Buffalo with the addition of Molasses, palm frond, palm leaf extract, and each other. The parameters observed were the activity of enzymes cellulase, laccase, lignin, and manganese peroxidase, as well as microbial identification. The results of statistical analysis showed that insignificant differences (p> 0.05) affect of all treatments for parametresthe activity of the enzymes cellulase 2.22-3.51 U/ml, laccase 10.62-20.11 U/ml, lignin peroxidase 1.74-4.93 U/ml, and manganese peroxidase 2.40-7.06 U/ml. The results of microbial identification found the bacteria Lactobacillus sp. Based on these, it was concluded that the microbes discovered in the local microorganism solution live because of the study environment, not the microbes inherited from the rumen's contents.
Keyword: Enzime activity, microbial morphology, enzyme sellulose, enzyme lignase,rumen microbes
| Item Type: | Article |
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| Subjects: | Agriculture > S Agriculture (General) |
| Depositing User: | Unnamed user with email admin@pub.eprints |
| Date Deposited: | 27 Feb 2023 01:31 |
| Last Modified: | 27 Feb 2023 01:39 |
| URI: | http://repository.ummy.ac.id/id/eprint/121 |
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